Cloned (Comment) | Organism |
---|---|
expression of p85 constructus from a retroviral vector | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deficiency of PI3-K regulatory subunit p85alpha in vivo results in a significantly greater number of trabeculae and significantly lower spacing between trabeculae as well as increased bone mass in both males and females compared to their sex-matched wild-type controls. p85-/- osteoclast progenitors show impaired growth and differentiation, which is associated with reduced activation of Akt and mitogen-activated protein kinase Erk1/Erk2 in vitro, and a significant reduction in the ability of p85-/- osteoclasts to adhere to as well as to migrate via integrin alphanybeta3. Restoring the expression of the full-length form of p85alpha but not the version with a deletion of the Src homology-3 domain restores the maturation of p85-/- osteoclasts to wild-type levels, overview. Phenotypes, overview | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + phosphatidylinositol-4,5-bisphosphate | Homo sapiens | - |
ADP + phosphatidylinositol-3,4,5-trisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone | - |
Homo sapiens | - |
osteoclast | osteoclasts express multiple regulatory subunits of class IA PI3-K, although the expression of the full-length form of p85alpha is most abundant | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + phosphatidylinositol-4,5-bisphosphate | - |
Homo sapiens | ADP + phosphatidylinositol-3,4,5-trisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | regulatory p85alpha and p85beta subunits of class IA PI3-K share near identity in the functional domains in the C-terminus, including the amino-SH2 and the carboxy-SH2 domains, which are critical for mediating association with other SH2-containing proteins as well as binding to the p110 catalytic subunit | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
class IA phosphatidylinositol 3-kinase | - |
Homo sapiens |
PI3-K | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | SHIP downregulates PI3-K-initiated signals by dephosphorylating PI-3,4,5-triphosphate | Homo sapiens |
physiological function | the p85alpha subunit of class IA phosphatidylinositol 3-kinase regulates the expression of multiple genes involved in osteoclast maturation and migration | Homo sapiens |